SIMULTANEOUS LOADING OF PCR-BASED MULTIPLE FRAGMENTS ON MOUSE ARTIFICIAL CHROMOSOME VECTORS IN DT40 CELL FOR GENE DELIVERY

Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery

Abstract Homology-directed repair-mediated knock-in (HDR-KI) in combination with CRISPR-Cas9-mediated double strand break (DSB) leads to high frequency of site-specific HDR-KI.While this characteristic is advantageous for generating genetically modified cellular and animal models, HDR-KI efficiency in mammalian cells remains low.Since avian DT40 ce

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Testing Vegetation Flammability: The Problem of Extremely Low Ignition Frequency and Overall Flammability Score

In the recent decades changes in fire regimes led to higher vulnerability of fire prone ecosystems, with vegetation being the only component influencing fire regime which can be managed in order feline 1-hcpch vaccine to reduce probability of extreme fire events.For these management practices to be effective reliable information on the vegetation f

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